UV analyzer manufacturers supply, [Washington] ultraviolet analyzer controlling prices, reliable quality UV analyzer is the application of fluorescence techniques, fluorescence techniques, what is it? First, look at what is fluorescence, also for "fluorescent" refers to a light-emitting cold light phenomenon. When the temperature of a substance by a wavelength of incident light (usually ultraviolet light or X-rays) radiation, absorption of light energy into the excited state, and immediately de-excitation and the wavelength of the incident light emit longer than the outgoing light (typically wavelengths visible light); And once stopped the incident, luminous phenomenon also will immediately disappear. Having such properties on the outgoing light is called fluorescence. Know what is fluorescence, can think what the name suggests fluorescence technique. Fluorescence of certain substances is subject to certain wavelengths of light excitation, in a very short period of time (10-8 seconds) emit light of a wavelength greater than the excitation wavelength, such light is called fluorescence. The luminescence phenomenon in all aspects of the application and the method is called fluorescence (fluorescent technique). Substance emitting fluorescence by ultraviolet irradiation of the phenomenon can be divided into two cases, the first one is the spontaneous fluorescence, such as chlorophyll, heme by ultraviolet irradiation, to red fluorescence, called autofluorescence; second is induced fluorescence, the fluorescent dye by the object and then emit fluorescence by ultraviolet irradiation, called induced fluorescence. Fluorescence techniques in biochemistry and molecular biology research applications include the following aspects: 1, material qualitative: Different phosphors have different excitation and emission spectra, fluorescence therefore be used to identify substances. Compared with absorption spectrometry, fluorescence method has a higher selectivity. 2, the quantitative determination of: the use of lower concentrations in the fluorescence intensity of the sample is proportional to the relationship between the concentration of the sample can be quantitatively analyzed the content of the fluorescent components, commonly used in the determination of amino acid, protein and nucleic acid content. Quantitative determination of fluorescence is an advantage of high sensitivity, such as the determination of vitamin B2 limited up to 1 ng / ml, the advantage needed when the measurement sample is greatly reduced. This method can also be applied to the quantitative determination of enzymatic reactions, provided that the reaction before and after the change in fluorescence intensity, can be used to determine the content of the enzyme and the enzyme reaction rate, etc.. 3, the study of the physical and chemical properties of biological macromolecules and molecular structure and conformation: Fluorescence excitation and emission spectra, quantum yield and fluorescence lifetime and other parameters only and intramolecular fluorescence chromophore structure itself, but also on strongly dependent on the environment around the chromophore, which is very sensitive to the surrounding environment. Take advantage of this feature by measuring the change in fluorescence parameters related to study the site where the fluorescent chromophore microenvironment features and changes. In this study, in addition to the use of biological macromolecules itself fluorescent chromophore (such as tryptophan, tyrosine, guanosine acid, such fluorescence is called the intrinsic fluorescence) than can be some special fluorescent dye molecules covalently bound or adsorbed on a certain part of biomolecules by measuring the fluorescence of the dye molecule changes of biological macromolecules, the dye molecules are called "fluorescent probe", they emit fluorescence generally called as exogenous fluorescence. The application of fluorescent probes, greatly expand the fluorescence technique in molecular biology applications. 4, fluorescence lifetime, quantum yield and other parameters can be studied in the energy transfer of biological macromolecules phenomenon: by this phenomena can be obtained information about the interior of the biological macromolecules. Contact: Fanmei Tel :0550 -7,092,555 Mobile: 13855057418